Hi, Tojo107.
I know these guys are trying to help, even though they aren't really answering the question the way you intended.
1. Yes, it will fire with no spring & guide rod. You don't even have to hold the slide in place. Push it forward all the way, let it go, pull the trigger, bang. The slide will recoil just like always, but won't go back forward again. Same recoil in the hand, it doesn't hurt you or the gun. Put another round in chamber and push slide forward, and yes it will shoot again. This is what you're trying to avoid, isn't it?
2. So what can you do? Remove the slide stop and take it with you. The slide stop is what locks the barrel in battery when the slide is all the way forward. Without it, the barrel won't cam up into position and lock. When you take the barrel out, look at that slotted foot at the bottom (the bottom lug). That is where the slide stop pin would push the barrel upward and lock it into battery. Without the pin, no lockup.
3. While you're at it, why not take the barrel with you? It fits in the pocket. Or just take the slide stop.
Hope that helps.